Journal: Viruses

Article Title: Cap Is the Protease of the Porcine Circovirus 2

doi: 10.3390/v14071550

Figure Lengend Snippet: The protein-protein interactions (PPIs) network analysis using STRING database. The linkers indicate associations of function and physical protein. The thickness of line indicates the strength of correlation (PPI enrichment p value < 0.05). Proteins represented have been named as NCBI gene names. JMJD6 and CCT5 were marked with Stars.

Article Snippet: The gene cap, JMJD6 and CCT5 were synthesized by GenScript (Nanjing, China).

Techniques: Protein-Protein interactions

Journal: Viruses

Article Title: Cap Is the Protease of the Porcine Circovirus 2

doi: 10.3390/v14071550

Figure Lengend Snippet: We validated the PCV2 Cap down-regulated host proteins by Western blotting (WB). ( A ) The expression of JMJD6 in PK-15 cells was determined by WB analysis. ( B ) The expression of CCT5 in PK-15 cells was determined by WB analysis.

Article Snippet: The gene cap, JMJD6 and CCT5 were synthesized by GenScript (Nanjing, China).

Techniques: Western Blot, Expressing

Journal: Viruses

Article Title: Cap Is the Protease of the Porcine Circovirus 2

doi: 10.3390/v14071550

Figure Lengend Snippet: Expression and purification of Cap, JMJD6 and CCT5. ( A ) SDS-PAGE analysis of the purified EDA-Cap. M, protein molecular mass standards; 1, the purified EDA-PCV2 Cap. ( B ) SDS-PAGE analysis of the purified JMJD6. M, protein molecular mass standards; 1, soluble fraction after cell sonication; 2, the purified JMJD6. ( C ) SDS-PAGE analysis of the purified CCT5. M, protein molecular mass standards; 1, soluble fraction after cell sonication; 2, the purified CCT5.

Article Snippet: The gene cap, JMJD6 and CCT5 were synthesized by GenScript (Nanjing, China).

Techniques: Expressing, Purification, SDS Page, Sonication

Journal: Viruses

Article Title: Cap Is the Protease of the Porcine Circovirus 2

doi: 10.3390/v14071550

Figure Lengend Snippet: The in vitro reaction assays and the PPI networks. ( A ) SDS-PAGE analysis the in vitro reaction that Cap digested JMJD6 and CCT5 at 37 °C 30 min, respectively. ( B ) Statistical analysis of the deduction of JMJD6 and CCT5 due to the digestion of Cap. All data were obtained from at least three independent experiments ( p < 0.05, p < 0.01, *** p < 0.001). ( C ) The PPI networks of the PCV2 Cap with JMJD6 and CCT5.

Article Snippet: The gene cap, JMJD6 and CCT5 were synthesized by GenScript (Nanjing, China).

Techniques: In Vitro, SDS Page